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The development of market gardening is faced with many problems among which there are the nematodes of the genus Meloidogyne. The aim of this study is twofold: (a) determining the Meloidogyne species that are crowned by the perineal plates of female and (b) studying the impact of the multiplication of these nematodes on the tomato cv Roma (Lycopersicon esculentum L). Root samples were collected at five sites around Bamako, Mali. In a trial, nematode larvae were extracted from the roots of culture and then inoculated on tomato plants. A second tomato plants non-inoculated was used as control. Using a maceration-filtration method, three Meloidogyne spp. were identified: Meloidogyne arenaria, Meloidogyne javanica and Meloidogyne incognita. All of the inoculated plants reacted by the presence of galls that were not observed on the non-inoculated plants.